EIDC
Wright, R.

Population dynamics of evolving bacterial populations in liquid media, 2020

https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4 Cite this dataset
This data was collected from co-evolving bacterial populations containing Pseudomonas fluorescens strain SBW25 and a plasmid, pQBR57. The composition of the community was tracked using flow cytometry to distinguish 1) an unlabelled wild type strain 2) a dTomato compensated host (SBW25 KO PFLU4242), and 3) a wild type host bearing a Green fluorescent protein (GFP) labelled compensated plasmid (pQBR57 KO 0059).
Publication date: 2022-11-23

Format

text

Spatial information

Study area
Spatial representation type
Tabular (text)
Spatial reference system
OSGB 1936 / British National Grid

Temporal information

Temporal extent
2020-01-01    to    2020-12-31

Provenance & quality

Flow cytometry data was filtered to remove background noise (using thresholds in forward scatter (FSC-H), side scatter (SSC-H) and blue signal (PB450-H) channels), then all events were classified by fluorescence (flow_data.txt) to provide a community composition for each sample recorded as population fractions (fraction_data.txt).

Correspondence/contact details

Wright, R.
University of Manchester
 rosanna.wright-2@manchester.ac.uk

Author

Wright, R.
University of Manchester
ORCID iD icon https://orcid.org/0000-0002-8095-8256

Other contacts

Rights holder
University of Manchester
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
INSPIRE theme
Environmental Monitoring Facilities
Keywords
co-evolution , plasmid compensation , pQBR57 , SBW25
Funding
Natural Environment Research Council Award: NE/R008825/2
Last updated
27 February 2024 16:15

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This dataset is available under the terms of the Open Government Licence

Download the data Supporting documentation

CITE AS: Wright, R. (2022). Population dynamics of evolving bacterial populations in liquid media, 2020. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4